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175189

UPLC-MS/MS profiling and antitumor activity of <i>Silene succulenta<i/> Forssk. Growing in Egypt

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Natural therapy

Abstract

Abstract
The present study aims to detect the phenolics and flavonoids constituents of Silene succulenta Forssk. belonging to Caryophyllaceae family, also screening of their antioxidant and cytotoxic activities. GC-MS analysis is one of our goals in this study. The phenolic and flavonoid profiles were carried out using Micro plate assay. In addition, in vitro antioxidant activity was performed using DPPH assay while the Cytotoxicity was summarized by SRB test. GC-MS analysis for n-hexane was carried out using gas chromatography-mass spectrometry. Also ultra-performance liquid chromatography coupled with hybrid triple time-of-flight mass spectrometry (UPLC-Triple TOF-MS/MS) was carried out to determine the different phytochemicals in total alcoholic extract. Total phenolic and flavonoids content in aerial parts of S. succulenta were found 20.86 mg/g and 8.06 mg/g respectively. Cytotoxicity of n-hexane fraction showed a high activity against Breast (MCF7) and Lung Carcinoma (A549) cell lines with IC50 15.5 and 22.6 µg /ml respectively. Total alcoholic extract and ethyl acetate fraction showed antioxidant activities with IC50 799.8 ± 45.45 and 749.9 ± 29.73 (ug/ ml) respectively. Finally, ninety six compounds were detected in n-hexane using GC-MS and comparison with standards. UPLC-Triple TOF-MS/MS showed the presence of different classes of secondary metabolites (flavonoids, coumarins, amino acids, anthocyanidines and alkaloids)

DOI

10.21608/aijpms.2021.57206.1039

Keywords

<i>Silene succulenta<i/>, Caryophyllaceae, Phytoecdysteroids, Triterpenoidal Saponins, Cytotoxicity

Authors

First Name

Sarah

Last Name

Mahmoud

MiddleName

-

Affiliation

Department of Medicinal and Aromatic plants, Desert Research Center, Cairo, Egypt

Email

sarahabadawy@yahoo.com

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

Hassan

MiddleName

-

Affiliation

Department of Medicinal and Aromatic plants, Desert Research Center, Cairo, Egypt

Email

ahmedhasan_81@yahoo.com

City

Cairo

Orcid

-

First Name

Salwa

Last Name

Abu El Wafa

MiddleName

A

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

salwaabuel-wafa.pharmg@azhar.edu.eg

City

Cairo

Orcid

0000-0001-7165-4077

First Name

Abd El-Salam

Last Name

Mohamed

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt

Email

abdelsalam876@yahoo.com

City

Cairo

Orcid

-

Volume

1

Article Issue

2

Related Issue

25720

Issue Date

2021-06-01

Receive Date

2021-01-08

Publish Date

2021-06-01

Page Start

58

Page End

62

Print ISSN

2735-4598

Online ISSN

2735-4601

Link

https://aijpms.journals.ekb.eg/article_175189.html

Detail API

https://aijpms.journals.ekb.eg/service?article_code=175189

Order

7

Type

Original research articles

Type Code

1,562

Publication Type

Journal

Publication Title

Azhar International Journal of Pharmaceutical and Medical Sciences

Publication Link

https://aijpms.journals.ekb.eg/

MainTitle

UPLC-MS/MS profiling and antitumor activity of <i>Silene succulenta<i/> Forssk. Growing in Egypt

Details

Type

Article

Created At

23 Jan 2023