Meningitis could be fatal or a cause serious of long-lasting side effects. It is imperative that pediatric nurses have the knowledge and training related to lumbar puncture to ensure a positive experience for children. Aim: To assess effect of instructional module on nursing pitfalls related to the management of children having lumbar puncture. Design: A quasi-experimental design. This study was conducted at Shebin El-Kom Fever Hospital. A convenient sample of seventy nurses working at the previously mentioned setting and all hospitalized children with meningitis who had lumbar puncture procedures. Instruments: Instrument one: knowledge about meningitis, lumbar puncture, and its nursing care for children structured interviewing questionnaire. Instrument two: Nurses practices observational checklist Results, Nurses had higher level of knowledge on posttest (30.41 ± 2.32), and follow-up (22.32 ±5.79.) than pretest (10.50 ±2.83.).Also, they had fewer nursing pitfalls related to lumbar puncture on posttest (0.33 ± 0.50), follow-up test (2.93 ±1.57) than pretest (4.29 ±0.85). Conclusion: It was concluded that nurses who received instructional module had higher level of knowledge as well as nursing practices regarding lumbar puncture for children with meningitis on post –test than pre- test. Also, they had fewer nursing pitfalls related to lumbar puncture procedure on posttest rather than on the pre& follow-up tests. Recommendations: A continuous on- service training program for nurses regarding the management of meningitis in children is required.