Background: Endotracheal intubation (EI) and mechanical ventilation (MV) are frequently used in pediatric patients to optimize gas exchange, breathing and patient comfort while minimizing ventilator-induced lung injury. Comfort management is a priority for patients in all settings. Purpose: To assess nurses' knowledge and practices regarding supportive care to promote comfort behavior in intubated pediatric patients. setting: The study was conducted at pediatric intensive care unit (PICU) in Menoufia University Hospital. Design: A descriptive research was utilized to conduct the study. Sample: A convenient sample of 33 nurses who were working in the previously mentioned setting were included in this study. Instruments: Two instruments were used. Instrument one was a structured interviewing questionnaire sheet. Instrument two was observational checklist for nurses' practice. Results: More than half of nurses (60.6%) had unsatisfactory knowledge about supportive care to promote comfort behavior in intubated pediatric patients.  Also, the majority of nurses had low level of practice regarding supportive care to promote comfort behavior in intubated pediatric patients. Conclusion: Nurses had unsatisfactory level of knowledge and low level of practices regarding supportive care to promote comfort behavior for intubated pediatric patients. Recommendation: The study recommended that there was a serious need for improvement of nurses' knowledge and practices in relation to promoting comfort behavior in intubated pediatric patients.