Tramadol classified as a weak opioid with an analgesic effect similar to that of codeine and indicated for the treatment of moderate and sever pains The purpose of this study was to assess of non-medical use of tramadol among university students. Research design: A descriptive research design was utilized in this study. Setting: This study was conducted at 50% of Faculties of Benha University it included 7 Faculties. The sample: stratified random sample was used in this study which included 10% of students in the first year academic year (2016- 2017) number of students who shared in the study was 315 male students. Two instruments were used I): A structured interviewing questionnaire and included two parts. part 1): included (a): socio-demographic characteristics of students and their family characteristics, (b): psychological distress of the students. (c): Physical health of students part2): to assess knowledge of students about tramadol.  II): Likert scales consist of 3 level scales which measured attitude of students toward non- medical use of tramadol.  Results: Nearly more than three quarters of students (94.3%) didn't have tramadol trial. More than one third of students (33.2%) had good knowledge toward tramadol. More than three quarters of students (80.9%) had positive attitude toward tramadol. Conclusion: There were statistically significant relations between student's family characteristics and tramadol trial. While there was highly statistical significant relations between health status and tramadol trial. there was a positive association among student's total knowledge and total attitude with their characteristics. Recommendations: health education programs for all students in the university regarding non-medical use of tramadol