Osteoporosis is a progressive skeletal disease that affects hundreds of millions of people worldwide, predominantly postmenopausal women. The aim of this study: was to assess the awareness of working women toward osteoporosis. Research design: A descriptive research design was utilized in this study. Setting: This study was conducted in Benha University Administration. The sample: A simple random sample was used to choose 200 women. Two instruments were used. Instrument one: Social characteristics of the sample structured interview questionnaire, medical history, knowledge andpractices of the studied sample. Instrument two: Osteoporosis risk factors checklist. Results: ages of 37% of the studied women from 30-40 years old, women who had no knowledge about osteoporosis were 41%, women who had satisfactory practices were 69.5%. Conclusion: many women had poor total knowledge about osteoporosis and poo total practice.  Recommendations: Health educational program should be developed and implemented for all women to increase their awareness regarding prevention of osteoporosis.