Gynecological examination is crucial for protecting the reproductive health of women. The purpose of this study was to assess knowledge, practice and attitude regarding gynecological examination among maternity nurses at banha university hospital. The study was conducted in obstetrics and gynecology department at banha university hospital.  The design of this study was a descriptive research design. A convenient sample included 50 nurses were recruited in the study. Three instruments were utilized for data collection, interviewing questionnaire sheet, observation check list and modified Likert scale. The result of this study revealed that 28% of studied nurses had adequate knowledge, 34% of them had positive attitude and 48% of them had competent performance regarding gynecological examination. Conclusion, this study concluded that less than three quarters of studied nurses had inadequate knowledge regarding gynecological examination and less than half of studied nurses had uncertain attitude, also more than half of studied nurses had incompetent performance of clinical gynecological examination. Therefore, it is recommended that national educational programs and workshops should be designed to promote knowledge, practices and attitudes of maternity nurses regarding gynecological examination.