Background: A stoma is a common procedure performed in children; mostly Colostomy in infancy and childhood is usually performed for benign disease and is of a temporary nature. Children are a high risk group for complications related to the stoma opening.  The main reason for the development of such complications is a lack of knowledge and skills related to stoma care therefore requires skilled care by the patient and or his parents. Purpose:  The purpose of this study was to assess caregivers' knowledge and practices regarding stoma care of their children in Menoufia University Hospital. Methods: A descriptive research design was conducted at Menoufia University hospital sampling: sixty primary caregivers and their children were included. The data was collected using knowledge and practice forms regarding stoma care.  Results: The findings revealed that all of caregivers (100%) had poor level of knowledge regarding colostomy and the maximum mean scores (14.63 ±5.7). Also, all of caregivers (100%) had poor practice level (M±SD=1.67 ±1.03). Conclusion: Caregivers' knowledge and practice regarding stoma care of children having colostomy in Menoufia University Hospital are at on poor level. Recommendations: The results show that there is a need for the development of caregivers' knowledge and practice levels in relation to colostomy care.