The term of health-care waste includes the waste generated within health-care facilities, research centers and laboratories related to medical procedures. The purpose of this study was to assess knowledge and performance of healthcare providers regarding hospital waste management in Menoufia governorate hospitals. Design: An observational cross-sectional research design. A convenient sample of 360 staff nurses and 150 workers (Housekeepers and storage, final treatment unite and disposal workers) was selected to carry out this study. Setting; this study was conducted at three hospitals at Menoufia   governorate, (Shebin El-Kom Teaching Hospital, Menoufia University Hospital, and El-Helal Health Insurance Hospital). Instruments: Two Instruments were used. Self-administered questionnaire and participant observation checklist. Results: Majority of nurses (89.4%)   in the study settings had high level of knowledge and high performance about hospital waste management and the percentage was higher in Teaching hospital than El-Helal Health Insurance (80.0%.) and University hospital (88.3%.). Most workers of the study settings had high level of knowledge and performance especially in El-Helal Health Insurance hospital than Teaching hospital and University hospital. In addition there was statistically significant positive correlation between knowledge of workers and nurses about hospital waste management and their performance. Conclusion: There was positive correlation between knowledge of nurses and workers about hospital waste management and their performance and awareness .Recommendation; hospital administration should implement Mandatory training of Health Care Personnel by a competent authority , make sure that their employees have been trained  on hospital waste management system.