Professional standards are authoritative statements of the duties that school nurses should completely perform and aim to fulfillment the profession's obligation to provide a means for improving the quality of care. The purpose of the study was to assess the role of school nurses regarding professional standards in Benha City. Design: Descriptive research design was utilized to conduct the study. Setting: The present study was conducted in (109) Governmental Primary School. Sample A purposive sample of school nurses working in the previous settings were included (n= 100 school nurses). Instruments Two tools were used in this study. The first instrument: A structured interviewed questionnaire to assess school nurses, knowledge and their reported practices about professional standards. The second instrument was an observational checklist to assess the healthy school environment. Results 71.0% of the studied school nurses had a good knowledge of the professional standards and 56.0% of them had unsatisfactory practices. There was a positive statistical significant relation between total knowledge of school nurses and their practices regarding professional standards. Conclusion more than two thirds of school nurses had a good total knowledge score regarding school heath program and professional standards, while more than half of them had unsatisfactory total practices score. Recommendations: School nurses need in service training program about professional standards.