Evidence-based practice is considered a foundation for the provision of quality care. Nurse managers (NMs) play an important role promoting evidence-based practice (EBP) on clinical units within hospitals. However, there is a dearth of research focused on NMs perspectives about barriers to evidence-based nursing practice utilization. The purpose of the study was to assess the barriers to evidence-based nursing practice utilization from the perspective of nurse managers. A descriptive comparative design was used. The study was conducted at Menoufia University hospital and Shebin El-Kom Teaching hospital. A convenient sample was used to select 90 nurse managers. Data was collected using the perceived barriers questionnaire. Nurse managers reported that there barriers related to perceived value of research (e,g, lack of interest, 74.4%), personal (inadequate computer skills, 50.0%) and administrative (lack of knowledge about research, 66.70%). So, it was concluded that nurse managers were challenged by a large number of barriers It was recommended that the results of the study should be disseminated among the important key persons (nurse managers).