Cataract is the second cause of visual impairment and the first cause of blindness globally. So, inadequate nursing interventions lead to serious complications for patients with cataract surgery. Purpose of the study: was to examine the effect of a nursing intervention on nurses' knowledge and practice regarding cataract surgery. Design: a quasi-experimental research design was utilized. Setting: the current study was carried out at Ophthalmology Department in Menoufia University Hospital and El Ramad Hospital in Shebin El-Kom. Sample: a purposive sample of 25 nurses was included. Instruments: two instruments were used for data collection:  a self-administered questionnaire for nurses` knowledge and an observational checklist for nurse's practice. Results: The mean age for nurses was 36.8±8.94, all nurses were married females with more than 3 years of clinical experiences. There was an improvement in total mean score of nurses' knowledge from 23.60±2.87 pre intervention to 35.92±3.85 post intervention and after one week to 39.24±2.04 as a follow up intervention. Also, there was an improvement in the total nurse's practice score from 100.64±17.77 pre intervention to 244.56±23.65 post intervention and after one week to 250.96±20.86 as a follow up intervention. Conclusion: nurse's knowledge and practice improved after implementing nursing intervention. Recommendation: educational nursing interventions using the same educational methods should be carried out for nurses in ophthalmology settings to improve their knowledge and practices regarding cataract surgery.