Background: Nowadays workplace deviant behaviors considered one amongst the foremost serious issues facing today's organizations and its productivity as it goes against the legitimate interests of the organization. These behaviors can harm organizations or people in organizations. So, the study aimed to determine role of negative affectivity and workplace incivility on incidence of counterproductive work place behavior among nurses at Main Mansoura University Hospital. Method: A descriptive design was used and the study was conducted on 162 nursing staff working at Main Mansoura University hospital by using three tools as: Negative Affectivity questionnaire, workplace incivility questionnaire and counterproductive workplace behavior questionnaire. Results: the mean score of counterproductive workplace behavior is lowest (1.23±0.26) followed by workplace incivility(1.32±0.35) and negative affectivity(1.89±0.78). There was statistically significant positive correlation between counterproductive workplace behavior and both negative affectivity and workplace incivility (P<0.05) among nursing staff (n=162). Recommendation: design and implement policies to reduce the deviant behaviors in the hospital environment . Organizations need to adopt zero tolerance policies that clearly articulate the types of conduct are not acceptable. These policies need to be clearly defined and communicated to all in the hospital and all who enter. There needs to be channels for employees to communicate issues. There also needs to be commitment to upholding these policies from top management.