Background: Hospital procured contaminations are a noteworthy trouble for all hospital staff. Knowledge of health staff about health services infection and its transmission with counteractive action can limit the spread of illness in health care institutions and in Society, so staff nurse must be instructed about fundamental standards of disease control and ability on the grounds that the nature of nursing consideration relies on upon a substantial degree on the learning. The aim of the present study was to investigate the effect of nursing guidelines regarding infection control precautions on the knowledge of staff nurse in surgical units at Gastroenterology Center Mansoura University. Methods: A quasi- experimental design was used .Tool: two tool were applied tool1: personal data of nursing staff such as: name, age, educational level, marital status, years of experience, tool 2: Nurses knowledge interview sheet include; hand hygiene, Personal protective equipment, handling sharp instrument, environmental hygiene, urinary catheter, caring of wound dressing, waste management . Results: The studied nurses age was ranged from 18–30 years, the most educational level among them was technical diploma, about their previous years of experience it was ranged from 4-6 years. The applied infection control guidelines were found to be highly effective, regarding nurse's knowledge. Furthermore, the differences between nurses knowledge were statistically significant post guidelines compared to pre, immediate. Conclusion: Applying the infection control guidelines improve nurses' knowledge and continues nursing education should be applied as policy or hospital standard this help in applying universal precaution well , so decrease hospital cost
,safe patient health , decrease hospital stay .