Aim of the study is to investigate the utilization of self care guideline on promoting early initiation of breastfeeding among mothers in the delivery room. Research design Quasi experimental study design was used. Sample: A purposive sample included 100 pregnant mother.Setting: the study was conducted at antenatal and labor units at Benha University Hospital. Tool of data collection; an interviewing questionnaire sheet, observational checklist, follow up card and satisfaction sheet. Results: of the present study revealed that There was highly statistically significant difference in relation to all items concerning mother's knowledge pre and post intervention Majority of mothers were satisfied about self-care Guideline. There was a highly significant improvement of mother's practices in all self-care items during the postnatal period. Sixty five percent of mothers were initiated breastfeeding immediately after delivery. There was a highly significant difference between mother's knowledge and educational levels of mothers in the pre and post intervention period. Also, this study showed that, highly significant difference between mother's selfcare practice regarding initiation of breastfeeding and their residence, correct knowledge pre and post intervention and their satisfaction in the post intervention period. Conclusions: utilizing self-care guideline is significantly improves mother's knowledge and practices regarding early initiation of breastfeeding during the postnatal period, the majority of mothers were satisfied with utilization of self-care guideline. Recommendations: Integrated self care concept into the maternal and newborn health nursing under and post graduate student faculty of nursing Benha university and distributing brochure about self care for early initiation of breast feeding during antenatal care to all pregnant mothers.