Background: Hepatitis C virus is a chronic medical condition that has broad implications for a person's physical and mental wellbeing. In order for people with hepatitis C virus (HCV) infection to effectively deal with this condition, it is vital that they get great therapeutic care and support from many different sources as possible medical professionals, family and friends, and others living with hepatitis C. The purpose of this study was to evaluate the impact of psychosocial intervention on the quality of life in patients with chronic hepatitis C virus infection. Methods: A quasi-experimental study was conducted in this study. Results: The experimental and control groups were the same concerning the effective factors on the quality of life, such as age, sex, etc (P > 0.05). There was no significant difference between QOL mean score of both groups before the intervention, however the QoL significantly improved in the experimental group after the intervention (P= 0.001), while the QoL decreased in control group. Conclusion: The results of this study confirmed the beneficial effects of psychosocial intervention on the health related quality of life. Therefore, all hepatitis C patients should be counseled and encouraged to participate in educational programs at the time of diagnosis to reduce unnecessary behavioral changes and stigmatization perceptions to improve quality of life.