Intestinal stoma is a surgical opening made by means of a hollow organ through the abdominal skin forming a mouth that is in contact with the external environment for the externalization of waste, secretions and/or feces. Nurses can take a key role in caring for children with a stoma, both pre and postoperatively and assisting parents, caregivers, in becoming confident and independent in the care of stoma by providing knowledge on a specific medical and surgical condition, the surgical procedure, stoma care, and pouching equipment choices. Therefore, the aim of this study was to assess the effect of an educational program for mothers regarding care of their children having intestinal stomas. The study included 40 mothers of children having intestinal stomas recruited over six month's period. The study was carried out at the inpatient pediatric surgery department in Mansoura University Children's Hospital (MUCH). Results: The results of this study revealed, that there was statistically significant difference of the studied mothers' knowledge & practices regarding care of their children having intestinal stomas pre/ post educational program. The study concluded that, there was a positive effect of the educational program in improving mothers' knowledge & practices regarding care for their children having intestinal stomas. Recommendations: refreshing periodical and educational programs for pediatric nurses are essential for upgrading the mothers' knowledge & practices regarding care of their children having intestinal stomas.