Hemodialysis nurses have an essential role in ensuring conformity to the hemodialysis standards and providing effective and safe patient care. Also, hemodialysis nurses play a vital role in providing efficient, coordinated, and focused patient care ,tasks that contribute to direct patient care are subdivided into three phases (the pre dialysis phase, the intradialytic phase and the termination phase). Education and training are two components of staff development that occur after an employee's orientation. Methodology: quasi experimental research design was conducted in the hemodialysis unit of International Mansoura Hospital (IMH). Socio demographic data & Nurses' Knowledge about nursing care standards of hemodialysis vascular access patients questionnaire sheet was designed after reviewing the related recent literature to assess nurses' knowledge. It contains 12 questions related to nursing care standards of hemodialysis vascular access. Performance checklist regarding hemodialysis vascular access care including 12 steps was designed. All available nurses (30) during the study period employed in hemodialysis unit with various age, qualifications, years of experience, different level of education, and willing to participate voluntarily. Results: The present study revealed that: (a) the total means knowledge score of nurses was increased after sessions. (b) The total mean practice score of nurses was increased after sessions. Conclusion: Nursing care standards recommendations are essential for nurses working at the hemodialysis unit. Nurse's level of education and years of experience was positive correlation with their knowledge and performance, but it was not statistically significant.