introduction & Aim of the work: Resurfacing a wound with new epithelium involves two important processes, proliferation and migration of keratinocytes. This study aimed to investigate the correlation between epidermal proliferating cell nuclear antigen and desmoplakin expressions during normal wound healing in mice. Material and methods : Thirty male albino mice were divid­ed randomly into 2 groups, control (n=6) left unwounded, and wound model group (n=24). Two full-thickness linear wounds (2 cm) were made on the shaved dorsum of the wound model group. At different time periods (1, 2, 4, 6 days) after wound, skin specimens including samples from the wounds were excised, pro­cessed as paraffin blocks, sectioned and immunostained with anti-PCNA and anti-desmoplakin (11-5F) anti­bodies. Results: During early process of wound healing, the cells at the margin of the wound proliferated and were pushed toward the surface in-order to migrate as evidenced by absence of desmop­lakin expression. Later, at wound margin desmoplakin expression reap­peared and proliferation of cells de­creased. The new epidermis in the floor of the wound formed the source of the cells that would cover the wound.