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127107

ULTRASTRUCTURAL STUDY AND CYTOKERATIN EXPRESSION OF NORMAL TYMPANIC MEMBRANE AND AFTER EUSTACHIAN TUBE OBSTRUCTION : AN EXPERIMENTAL STUDY

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Last updated: 04 Jan 2025

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Abstract

The main target of the present work is to study the ultrastructure and expression of cytokeratin 13' 16{CK13/16), as a marker of ceil pro­liferation, of tympanic membrane (TM) of normal guinea pigs and after Eus-tachian tube (ET) obstruction. The present study was conducted on 24 healthy guinea pigs. The right ET na-sopharyngeai orifice of all animals was obstructed by electrocauterlza-tion while the contraiaterat left ear served as controls. The animais were classified into three equai croups ac­cording to the time interval following ET obstruction either after two, four or eight weeks. The TMs of 12 animals were stained immunohistochemically for detection of CK13/16 and that or other 12 animals were subjected for electron microscopic study. The re-
suits showed that the pars tensa of control group is formed of outer epi­thelial layer, lamina propria and inner rnucosal layer. Obstruction of ET led to inflammatory changes in the form of hemorrhage and infilteration of the subepithelial and submucosal layers of the lamina propria with inflamma­tory cells. After eight weeks of ET obstruction, the epithelium showed increase thickness and hype rpro life ra­tion as detected by increase the ex­pression CK13/16 that was not re­stricted to the basa! cell layer but found in many suprabasal layer. Also, the dense network of collagen fibers found in the normal TM was de­stroyed with few fibers remaining. From the present work it can be con­cluded that the patency of ET is es­sential for the integrity of TM. Also, the increase of CK 13/16 pattern and 
 
 

DOI

10.21608/mjmu.2002.127107

Authors

First Name

Eman M.

Last Name

El-Nashar

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Affiliation

Departments of Histology and Cytology and Otorhinofaryngofogy*. Benha Faculty of Medicine - Zagazig University

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First Name

Mosad Y.

Last Name

Abd EI-Aziz

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Affiliation

Departments of Histology and Cytology and Otorhinofaryngofogy*. Benha Faculty of Medicine - Zagazig University

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Volume

31

Article Issue

2

Related Issue

18984

Issue Date

2002-12-01

Receive Date

2020-12-02

Publish Date

2002-12-01

Page Start

349

Page End

367

Print ISSN

1110-211X

Online ISSN

2735-3990

Link

https://mjmu.journals.ekb.eg/article_127107.html

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https://mjmu.journals.ekb.eg/service?article_code=127107

Order

11

Type

Research (original) articles

Type Code

1,453

Publication Type

Journal

Publication Title

Mansoura Medical Journal

Publication Link

https://mjmu.journals.ekb.eg/

MainTitle

ULTRASTRUCTURAL STUDY AND CYTOKERATIN EXPRESSION OF NORMAL TYMPANIC MEMBRANE AND AFTER EUSTACHIAN TUBE OBSTRUCTION : AN EXPERIMENTAL STUDY

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Article

Created At

23 Jan 2023