Nosocomial infection is the infec­tion acquired within a hospital in a pa­tient hospitalized for at least 72 hours without signs or symptoms of infection prior to this time. It is one of the major problems all over the world concern­ing the patients and every member in hospital. The continuing increase in the incidence of these infections rep­resents an important problem in many countries, since they are associated with a high degree of morbidity and mortality.
Aim of the work: This study was conducted to estimate the prevalence of pathogenas in different type of N.C.I with special reference to MRSA
as a causative agent of nosocomia! infection. In addition, genotyping of the isolates will be done to add pre­cise markers for the epidemiological investigations of MRSA.
Patients : This study was conduct­ed over a period of eight months start­ing from October 1998 till the end of May 1999 on all patients (36700) ad­mitted to Mansoura University Hospi­tal. Patients included in this study are those who acquired infection in the hospital after admission i.e. nosoco-mially infected patients (1296 pa­tients).