This study was conducted on 40 non pregnant women suffering from lower genital discomfort for detection of chlamydia trachomatis {C. trachom-atis) from their cervices by three dif­ferent laboratory tests (Giemsa stain, Direct immunofluorescent antibody "DFA" stain, and culture on BGM cells). We found that 62.5% of young females (16 - 25 years) had C. tra­chomatis while older ones (26 - 35
years and 36 - 54 years) had less in­fections (25% and 12.5% respective­ly). 75% of women from rural areas had C. trachomatis while 25% of those from urban areas had C. tra­chomatis. As regards to the symp­toms and signs, 50% of women with cervicitis had C. trachomatis. As re­gards to the laboratory methods, 8 cases (20%) were positive by Giemsa
stain, 10 cases (25%) were positive by DFA, and 13 cases (32.3%) were positive by culture method. As re­gards to sensitivity and specificity to culture method, Giemsa stain was 61.5%, 100%, and DFA were 61.5% and 92.5 respectively. We conclude that, tissue culture method is most sensitive and specific method and considered to be the gold standard test for detection of C. trachomatis, however, processing time and cost have decreased its use in clinical practice.