Background: Although metronidazole
is the drug of choice for treatment
of trichomoniasis, resistant Trichomonas
vaginalis isolates have
been
identified. Aim
of the study:
So,
the
aim of our study is to investigate
the
in vitro susceptibility of T. vaginalis
against metronidazole in our locality.
Methods: Vaginal swabs were
collected from 420 cases attending
the outpatients' clinics of the
gynecology department, Mansoura
university hospital. The patients
were subjected to wet mount smear
examination and Giemsa staining followed by culture on modified
Diamond's media. In vitro susceptibility
testing was done for 49 trichomonas
vaginalis positive cases to
determine
minimal lethal concentration
(MLC) of metronidazole in each
case.
Results: Most susceptible isolates
(34.7%) were dead at MLC 1 µg\ml
followed by MLC 2 µg\ml at which 8
(16.3%) isolates were killed, followed
by 6 (12.2%) isolates at 4 µg\ml, 7
isolates (14.2%) at 8 µg\ml, 4 isolates
(8.1%) at 16 µg\ml,
and 3
(6.1%)
isolates at 32 µg\ml
while in
resistant
isolates 2 were killed at
concentration
64 µg\ml
and 2 iso- Conclusion: In our locality, most
isolates were susceptible to low
dose of metronidazole (lower than or
equal 32 µg/ml)with few resistant isolates
that could be explained by development
of drug resistance in future.