objectives: to evaluate hepatoprotective
effect of captopril, valsartan
in
hepatic ischemia-reperfusion injury
by assessing nuclear factor kappa
B
(NF-κB)
and lipid peroxidation.
Method: Twenty four rats were
randomly divided into four groups,
sham-operated group (control
group), ischemia-reperfusion model
(I/R group), IR with pretreated captopril
7 days before surgery(CAP
group)
and IR with pretreated valsartan
7days before surgery(VAL group)
3h
after induction of IR. Assay of serum
liver enzymes alanine transaminase
(ALT), aspartate transaminase (AST), serum tumor necrosis factoralpha
(TNF- α)
and interleukin – beta
(IL-1β)
as well as superoxide dismutase
(SOD), malondialdehyde (MDA)
in
liver homogenates and liver NF-κB
immunohistochemical
analysis and
hepatic
pathology were evaluated in
sacrificed
rats after 3 hours of induction
of IR. Results: There was in IR group < br />decrease of SOD and increase of
MDA levels in liver homogenates 3
hours induction of IR. CAP and VAL
pretreated groups showed significant
increase of SOD and significant decrease
of MDA compared to IR
group < br /> after 3 hours of induction of IR. Serum liver enzymes AST and
ALT were significantly increase in IR
group compared to sham group.
CAP and VAL pretreated groups
showed significant decrease of AST
and ALT compared to IR group.
Serum cytokines TNF-α and IL-1β
were significantly increase in IR
group compared to sham group.
CAP and VAL pretreated groups
showed significant decrease of
TNF-α and IL-Iβ compared to IR
group. NF-κB expression in liver
cells (staining fraction) was significantly
increase in IR group compared
to sham group 3 hours after
induction
of IR. CAP and VAL pretreated
groups showed significant
decrease
of NF-κB
expression compared
to IR group. CAP-IR & VAL-IR
groups
caused significant decrease
in
combined score for liver morphology
compared to IR group.