Beta
ManageSearchChatDiscover
175696

ENHANCED PRODUCTION OF TRICHODERMA CELLULASES THROUGH RECONSTRUCTION OF CELLULASE GENES

Article

Last updated: 26 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Trichoderma fungi produce economically important cellulases that comprise three key enzymes; endoglucanases, exoglucanases, and β-glucosidases.  In this study, a total of 28 isolates from Nile Delta were analyzed. The objectives were: 1) species identification of isolates, 2) evaluation of strains' cellulases production in submerged (SmF) and solid state (SSF) fermentation systems, 3) improve strains' cellulases production by mutagenesis and 4) enhance production of strains' cellulases through protoplast fusion and genome shuffling. On the basis of morphological characterization and molecular identification by DNA sequences analysis of ITS region, isolates were identified to species level as strains. 11 strains were chosen because of their unique properties in cellulase screening tests and they were investigated for the production of cellulases in SmF and rice straw SSF cultures. According to detailed examinations of culture filtrates, four promising strains were selected and subjected to genetic improvement through mutagenesis. Nine produced mutants showed better cellulases production than their parents. Moreover, the protoplast fusion of the nine mutants produced stable fusants that showed a marked increase in cellulase production over their mutant parents and original wild-type parents.  Finally, genome shuffling of Trichoderma cells produced strains were higher than their original wild-type parents in cellulase production.

DOI

10.21608/mjab.2018.175696

Keywords

Trichoderma cellulases, endoglucanases, exoglucanases, β-glucosidases

Authors

First Name

Kh. A.

Last Name

El-Halafawy

MiddleName

-

Affiliation

Prof. of Genetics, Genetic Engineering and Biotechnology Research Institute, University of Sadat City.

Email

-

City

-

Orcid

-

First Name

Ragaa A.

Last Name

Eissa

MiddleName

-

Affiliation

prof. of Genetics, Fac. of Agric., Menoufia Univ.

Email

-

City

-

Orcid

-

First Name

H. A.

Last Name

Hamza

MiddleName

-

Affiliation

Prof. of Genetics, Res. Institute, Univ. of Sadat City.

Email

-

City

-

Orcid

-

First Name

A. I.

Last Name

Fahmi

MiddleName

-

Affiliation

prof. of Genetics, Fac. of Agric., Menoufia Univ.

Email

-

City

-

Orcid

-

Volume

3

Article Issue

1

Related Issue

25473

Issue Date

2018-10-01

Receive Date

2021-06-06

Publish Date

2018-10-01

Page Start

49

Page End

50

Print ISSN

2357-0768

Online ISSN

2735-3532

Article File

MJAB_Volume 3_Issue 1_Pages 49-50.pdf

PDF . 305.3kB

Link

https://mjab.journals.ekb.eg/article_175696.html

Detail API

https://mjab.journals.ekb.eg/service?article_code=175696

Order

6

Type

original papers

Type Code

1,460

Publication Type

Journal

Publication Title

Menoufia Journal of Agricultural Biotechnology

Publication Link

https://mjab.journals.ekb.eg/

MainTitle

ENHANCED PRODUCTION OF TRICHODERMA CELLULASES THROUGH RECONSTRUCTION OF CELLULASE GENES

Details

Type

Article

Created At

23 Jan 2023