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174860

GENETIC IMPROVEMENT OF SOME TRICHODERMA ISOLATES AS BIOLOGICAL CONTROL AGENT

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Last updated: 26 Dec 2024

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Abstract

The main objectives of this study were isolation and characterization of some Trichoderma isolates and selection of the best isolates as biocontrol agent. Also improving of these isolates by protoplast fusion and selection of the best strains produced from protoplast fusion. Accordingly, 25 isolates of Trichoderma were obtained and characterized on morphological and molecular bases. It was found that the predominate species in Menoufia governorate   was T. harzianum. These isolates showed high ability in inhibition of growth rate of phytopathogenic fungi. Also, they demonstrated different abilities in producing chitinase enzymes (cell wall degradation enzyme for phytopathogenic fungi). Five isolates were selected for improving by protoplast fusion and six isolates obtained from fusion were selected on the basis of their high abilities in hydrolyzing chitin. Also, these strains were better than their parents in inhibition growth rate of phytopathogenic fungi. In addition, they integrated high production of chitinase enzymes. In green house evaluation on faba bean plants, the obtained result showed that the plants treated with these strains and the pathogen were better in growth parameters than the plants treated by the pathogen only. Also, this treatment showed better growth parameters than the plants treated by parenteral isolates and the phytopathogen.   

DOI

10.21608/mjab.2019.174860

Keywords

morphological features, ITS and TEF1-α, Mycoparasitism, Chitinase, Exochitinase, Genetic Improvement, Protoplast Fusion

Authors

First Name

Ragaa A.

Last Name

Eissa

MiddleName

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Affiliation

Prof. of Genetics, Fac. of Agric., Menoufia Univ.

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First Name

A. I.

Last Name

Fahmi

MiddleName

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Affiliation

Prof. of Genetics, Fac. of Agric., Menoufia Univ.

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City

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Orcid

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First Name

A. M.

Last Name

El-Zanaty

MiddleName

-

Affiliation

Assistant prof. Genetics, Fac. of Agric., Menoufia Univ.

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Volume

4

Article Issue

3

Related Issue

25372

Issue Date

2019-06-01

Receive Date

2021-06-03

Publish Date

2019-06-01

Page Start

51

Page End

52

Print ISSN

2357-0768

Online ISSN

2735-3532

Article File

MJAB_Volume 4_Issue 3_Pages 51-52.pdf

PDF . 314.9kB

Link

https://mjab.journals.ekb.eg/article_174860.html

Detail API

https://mjab.journals.ekb.eg/service?article_code=174860

Order

4

Type

original papers

Type Code

1,460

Publication Type

Journal

Publication Title

Menoufia Journal of Agricultural Biotechnology

Publication Link

https://mjab.journals.ekb.eg/

MainTitle

GENETIC IMPROVEMENT OF SOME TRICHODERMA ISOLATES AS BIOLOGICAL CONTROL AGENT

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Article

Created At

23 Jan 2023