Beta
ManageSearchChatDiscover
170411

INVESTIGATION OF VIRULENT AND TOXIN GENES EXPRESSION IN SOME SALMONELLA SEROVARS

Article

Last updated: 26 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Samples collected from patient stool during their course of investigation at hospitals ministry of health, Egypt as well as solid and liquid food samples collected from local markets were examined for presence of Salmonella spp. Isolation and serological identification showed that 92.5% of collected samples contained Salmonella spp belonged to six serovars; Salmonella   , enteritidis, Newport, derby, senftenberg and Virchow. These six serovars were checked by PCR for presence of three genes involved in virulence and toxin production, invA, avrA and stn. The gene invA was detected in all serovars, while avrA and stn genes were found in only 4 serovars. In this regard, S.typhimurium    was found to harbour the three tested genes. When Salmonella  typhimurium  grew on normal synthetic medium and natural animal cells (Red blood; RBC and murine epithelial cells; IEC-6) and incubated separately at different temperatures 5, 25, 35 and 40 C for 60 h., they showed regular increase in growth rate with increasing temperature. Also, total RNA concentrations increased up to 24 h. then slightly decreased at each examined temperature specially at 25, 35 and 40 C, but the highest RNA concentration was observed at 40 C. indicating its preparedness in the form of metabolic and cellular activities to cope with temperature stress. Moreover, S. typhimurium seems to be more potent and showed increased ability to induce the expression of enterotoxin gene (stn) by the exposure of temperature elevation specially when grown on murine epithelial cells; IEC-6 comparing with synthetic media or red blood cells.

DOI

10.21608/mjab.2020.170411

Keywords

Salmonella, Virulent genes, invA gene, avrA gene, enterotoxin gene (stn)

Authors

First Name

A.

Last Name

Fekry

MiddleName

-

Affiliation

Ministry of Health Central Laboratories, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Fahim

MiddleName

-

Affiliation

Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.

Email

-

City

-

Orcid

-

First Name

Walaa

Last Name

Hussein

MiddleName

-

Affiliation

Genetics and Cytology Department , Genetic Engineering and Biotechnology Division, National Research Centre (Affiliation ID : 60014618), Dokki, Egypt.

Email

-

City

-

Orcid

-

First Name

Wafaa

Last Name

Hanafy

MiddleName

-

Affiliation

Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Elbeltagy

MiddleName

-

Affiliation

Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.

Email

-

City

-

Orcid

-

Volume

5

Article Issue

2

Related Issue

24840

Issue Date

2020-04-01

Receive Date

2021-05-17

Publish Date

2020-04-01

Page Start

67

Page End

81

Print ISSN

2357-0768

Online ISSN

2735-3532

Article File

MJAB_Volume 5_Issue 2_Pages 67-81.pdf

PDF . 575.4kB

Link

https://mjab.journals.ekb.eg/article_170411.html

Detail API

https://mjab.journals.ekb.eg/service?article_code=170411

Order

2

Type

original papers

Type Code

1,460

Publication Type

Journal

Publication Title

Menoufia Journal of Agricultural Biotechnology

Publication Link

https://mjab.journals.ekb.eg/

MainTitle

INVESTIGATION OF VIRULENT AND TOXIN GENES EXPRESSION IN SOME SALMONELLA SEROVARS

Details

Type

Article

Created At

23 Jan 2023