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170407

IMPACT OF ENDOPHYTIC LIPOPEPTIDES USE AGAINST SOME PLANT PATHOGENIC MICROORGANISMS

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Last updated: 26 Dec 2024

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Abstract

Twelve dissimilar plant associated bacterial colonies were isolated from five different tomato seedlings, the Non Ribosomal Lipopeptides genes were detected by degenerated primers technique in the twelve tomato endophytic isolates, only the long rode bacilli endophytic strains were found to harbour the lipopeptides synthetases genes, these three strains were given laboratory names of (BMG100, BMG101 and BMG102) for further pursuit. Partial sequencing of 16s RNA gene and protein profile analysis for the three bacilli isolates showed that all isolates belonged to Bacillus subtilis. The lipopeptides production by HPLC analysis revealed that all the endophytic isolates from tomato stems had the ability to produce lipopeptides except the third isolate named B. subtilis BMG102 showed no production. different levels of volumetric oxygen transfer coefficient (KLa) were used as cursors for lipopeptides production. The maximum concentration of surfactin type was about 1431, 529 and 437 mg.L-1, the plipastitin (fengycin type )  was 360 198 mg.L-1  and zero while the  Mycosubtilin (iturin type) was zero, 228 and 414 mg.L-1 for the strains B. subtilis ATCC21332, BMG100 and BMG101, respectively. Surfactin production is visibly optimal at high oxygen transfer (kLa) and showed more than 0.07 S-1, while the optimum production of the fengycin and iturin could be observed at mild values of O2 transfer (kLa) which equal 0.01 and 0.015 S-1 respectively. The antibacterial - antifungal effects of the three B.subtilis extracts collected during  lipopeptides productivity regimes showed inhibition activity against Pseudomonas syringae, Ralstonia solanacearum and Erwinia amylovora, as will as the antifungal activity against Aspergillus niger, Phanerochaete chrysosporium and Alternaria alternata. These results indicate potential use of B.subtilis strains as biocontrol agents, especially when considering commercial implementation. Studies regarding the lipopeptides applications, should also be considered as this will influence the efficacy of the lipopeptides against the target organisms.

DOI

10.21608/mjab.2020.170407

Keywords

Endophytic, Lipopeptides, plant Pathogens, biocontrol agents

Authors

First Name

A.

Last Name

Mandour

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Affiliation

Ministry of Health, Shebin El kanater Hospitaly, Egypt.

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Orcid

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First Name

S.

Last Name

Fahim

MiddleName

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Affiliation

Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.

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City

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Orcid

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First Name

Walaa

Last Name

Hussein

MiddleName

-

Affiliation

Genetics and Cytology Department , Genetic Engineering and Biotechnology Division, National Research Centre (Affiliation ID : 60014618), Dokki, Egypt.

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Orcid

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First Name

Wafaa

Last Name

Hanafy

MiddleName

-

Affiliation

Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.

Email

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City

-

Orcid

-

First Name

A.

Last Name

Elbeltagy

MiddleName

-

Affiliation

Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.

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Volume

5

Article Issue

2

Related Issue

24840

Issue Date

2020-04-01

Receive Date

2021-05-17

Publish Date

2020-04-01

Page Start

43

Page End

65

Print ISSN

2357-0768

Online ISSN

2735-3532

Article File

MJAB_Volume 5_Issue 2_Pages 43-65.pdf

PDF . 673.4kB

Link

https://mjab.journals.ekb.eg/article_170407.html

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https://mjab.journals.ekb.eg/service?article_code=170407

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original papers

Type Code

1,460

Publication Type

Journal

Publication Title

Menoufia Journal of Agricultural Biotechnology

Publication Link

https://mjab.journals.ekb.eg/

MainTitle

IMPACT OF ENDOPHYTIC LIPOPEPTIDES USE AGAINST SOME PLANT PATHOGENIC MICROORGANISMS

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Article

Created At

23 Jan 2023