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169625

EVALUATION THE ANTIMICROBIAL ACTIVITY OF THYME AND ROSEMARY EXTRACTS AGAINST SOME FOOD RELATED BACTERIA

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Last updated: 04 Jan 2025

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Abstract

Today, the increased use of antibiotics leads to the incidence of resistant strains. We are faced with lack of new antimicrobial drugs that have fewer side effects than antibiotic. Plants are the main source of secondary metabolites, which can use as natural antimicrobial agents. In current study, rosemary and thyme were used to determine their chemical composition, phenolic compounds profile (by HPLC) and evaluate the antimicrobial effects against some pathogenic bacteria. Ethanol and acetone extracts of rosemary and thyme leaves (with concentrations of 50, 100, 200 and 400 mg/ml) were prepared, and antibacterial activities were tested by disc diffusion method on strains of Gram-positive bacteria: Staphylococcus aureus, Bacillus cereus and Streptococcus pyogenes. Gram-negative bacteria: Escherichia coli, Salmonella typhi and Shigella species. The results showed that all the concentrations of ethanolic and acetone rosemary extracts showed the highest level inhibitory zone diameters against Escherichia coli and Bacillus cereus, respectively. While the same extracts recorded the lowest level inhibitory zone against Staphylococcus aureus for both extracts. Similarly, all concentrations of the ethanolic and acetone thyme extracts showed the highest level inhibitory zone diameters against Escherichia coli and Salmonella typhi respectively, while the same extracts recorded the lowest level inhibitory zone diameters against Bacillus cereus and Escherichia coli, respectively. we can recommend the use of both Thyme and Rosemary as natural antimicrobials agents against food spoilage bacteria to overcome the phenomenon of antibiotics resistance, which represents a serious challenge to humans.

DOI

10.21608/mjab.2021.169625

Keywords

thyme, rosemary, Pathogenic bacteria, phenolic compounds (HPL)

Authors

First Name

W. S. Abd El

Last Name

Kader

MiddleName

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Affiliation

Ministry of Health and population

Email

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City

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Orcid

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First Name

A. A.

Last Name

Sakr

MiddleName

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Affiliation

Biochemistry department, Faculty of Agriculture, Menofia University.

Email

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City

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Orcid

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First Name

Kh. M.

Last Name

Taha

MiddleName

-

Affiliation

Biochemistry department, Faculty of Agriculture, Menofia University.

Email

-

City

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Orcid

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First Name

M. M.

Last Name

Abozid

MiddleName

-

Affiliation

Biochemistry department, Faculty of Agriculture, Menofia University

Email

-

City

-

Orcid

-

Volume

6

Article Issue

3

Related Issue

24746

Issue Date

2021-04-01

Receive Date

2021-05-09

Publish Date

2021-04-01

Page Start

29

Page End

40

Print ISSN

2357-0768

Online ISSN

2735-3532

Article File

MJAB_Volume 6_Issue 3_Pages 29-40.pdf

PDF . 529.4kB

Link

https://mjab.journals.ekb.eg/article_169625.html

Detail API

https://mjab.journals.ekb.eg/service?article_code=169625

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Type

original papers

Type Code

1,460

Publication Type

Journal

Publication Title

Menoufia Journal of Agricultural Biotechnology

Publication Link

https://mjab.journals.ekb.eg/

MainTitle

EVALUATION THE ANTIMICROBIAL ACTIVITY OF THYME AND ROSEMARY EXTRACTS AGAINST SOME FOOD RELATED BACTERIA

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Article

Created At

23 Jan 2023