This research represents the first bio-geography study of soil bacteria in Kuwait, by analyzing the 16S rRNA gene in 53 isolated bacteria collected from the six Kuwaiti governorates in summer and winter seasons. No significant differences were found in bacterial communities among seasons, indicating that seasonal variation in temperature had little influence on bacterial community. The isolated soil bacteria were studied using phylogenetic analysis, that help in building up the genetic database of the bacterial Kuwaiti isolates. Strains of soil bacteria from different soil samples of the six Kuwaiti governments were isolated. Twenty-eight bacterial taxa were identified by 16S rRNA gene sequencing. The results showed that most of bacteria species belong to Bacilli., while Gammaproteobacteria, Actinobacteria and Betaproteobacteria are less abundant. Cronobacter sakazakii, Enterobacter cloacae, Stenotrophomonas pavanii, Serratia rubidaea, Mixta theicola, and three different species of Pseudomonas were detected. No significant seasonal differences were found. Whole genome sequencing is required to understand how these taxa might be adapted to their different environments.