Chronic renal failure (CRF) is associated with male gonadal dysfunction that may lead to impotence, reduced libido, reduced size of testes and impaired spermatogenesis. The present study assesses the protective impact of Raphanus sativus seed extract (RSE) on testicular dysfunction and infertility associated with CRF induced by adenine in male rats. Twenty adult male albino rats were divided into four groups (5 each) and received their treatment for 4 weeks: group I, control; group II, (RSE): (0.52 ml/kg) orally via stomach tube, group III, (AD): (0.75 % W/W in diet), group IV, (RSE+ AD): received RSE and AD the same dose and route as groups II and III daily. The results showed a significant decline in the body weight gain, testicles and epididymides weights, testosterone level, semen quality ( sperm count, motility, and viability), reduced glutathione (GSH) level, superoxide dismutase (SOD) and catalase (CAT) activities, and increase in kidney weight, serum creatinine (SCr), BUN, uric acid, FSH & LH levels, sperm abnormality, malondialdehyde (MDA) & nitric oxide (NO) in AD treated rats, and histopathological alteration in kidney and testes tissue. But, RSE and AD co-administration partially successfully blocked these parameters and prevent histological structures abnormalities that nearly remain within normal levels in comparison with AD group < strong>.