Aquatic organisms, including fish, accumulate pollutants directly from contaminated water and indirectly via the food chain which probably may cause variation in the way these pollutants affect different trophic level species. Blood parameters have been recognized as valuable tools for monitoring fish health. Haematological and serum biochemical parameters were studied and compared in different trophic level fish species of Parachanna obscura (carnivores), Clarias gariepinus (omnivores) and Oreochromis niloticus (herbivores). Blood parameter revealed low PCV in Oreochreomis niloticus (16.70±1.15%) which significantly varies (P<0.05) with value (23.40±0.58% and 21.33±1.76%) recorded for Clarias gariepinus and Parachanna obscura respectively. The highest values of Hb (7.90g/dl) were obtained in Clarias gariepinus, this also reflected on white blood cell and red blood cell count. There were significant differences in the values observed in MCH, MCHC and MCV for species. Most biochemical parameter increases along the feeding habit from carnivorous to omnivorous i.e. Parachanna obscura > Clarias gariepinus >Oreochromis niloticus. Based on these results, it appears that there are variations in the blood profile of fish as regard their feeding habit as well as physiological effects of anthropogenic activities on fish.