Quercetin (Qu.) is considered as one of the flavonoids that were known by their anti-inflammatory and anti-carcinogenic effects. Even though, its implication in clinical trials of cancers is still limited. This study aims to determine the effect of Qu. against progression of Liver cancer using an in vitro model of hepatocellular carcinoma. Hepatocellular carcinoma cell line HepG2 was treated with serial concentrations of Qu. and the MTT assay data indicated a significant dose dependent effect of Qu. in lessen the cell proliferation rate in a dose dependent manner. This effect on cell proliferation was confirmed by the significant ceasing of cell cycle at G1/S phase upon Qu. treatment. The wound healing assay illustrated the inhibitory effect of Qu. on the capability of HepG2 to migrate to the induced wound. Finally, Qu. significantly induced HepG2 cell apoptosis via the significant upregulation of proapoptotic Bax expression and downregulation of antiapoptotic Bcl2. In conclusion, Qu. showed potent anti-cancer characteristics that been indicated by its prominent effect on HepG2 cells proliferation, motility, and survival.