The provision of transverse openings in floor beams to facilitate the passage of utility pipes and service ducts results not only in a more systematic layout of pipes and ducts; but also translates into substantial economic savings in the construction of a multi-storey building. Over the past several decades, many researchers exerted great efforts to predict and interpret the behavior of beams with web openings. Eleven beams were tested under static loading up to failure, ten of them were simulated the negative moment regions of reinforced concrete T- beams, were fabricated with large opening through the web and the other beam has solid web. In this study the effect of openings depth, openings length and the distance between the nearest support to the opening and its center is investigated. These beams made from high strength concrete of 90 MPa. The pattern of cracks and modes of failure were observed. The concrete strain and reinforced steel strain around openings were recorded. The maximum midspan deflection, at inner edge of opening and difference between maximum deflections of two edges of the opening were measured. The cracking and ultimate loads as well as crack width were measured. The results were given in shape of photos, tables and curves.