There is an increased interest to understand and evaluate travel patterns and behaviors, as an attempt to understand travel choices and to identify priorities for interventions. Factors affecting travel choices and behavior were divided into two main groups: non-urban factors and urban factors. This study examined the effect of some various land-use factors on the behavior of the inhabitants of Assiut city-Egypt and measured the preferences of users for special and alternative modes of mechanical mobility in an attempt to determine the required and urgent development priorities to improve transportation services and change travel behaviors toward sustainability. The study was based on an analytical descriptive method. The database was derived from a field study of Assiut city during the period (January 2017 to March 2017). The data were collected through: urban survey of the urban environment supporting the mobility style and behavior of the respondents, in different neighborhoods in Assiut city. During the same period, the researchers distributed a questionnaire at work centers, educational services and some commercial services sites in Assiut. The relative weights for factors limiting the use of alternative means by individuals as well as factors encouraging individuals to use alternative means of transport were determined. The study concluded that mixed land use alone is not enough to reduce mobility and to encourage individuals to move without using mechanical or motorized ways. It should be accompanied with a balanced diversity distribution of services with access by multiple means of transport. In addition, the use of mixed and diverse land use and the shortness of travel distance has a stronger impact on the quality and efficiency of pedestrian corridors and their transit areas on increasing pedestrian mobility, while the quality and efficiency of the cycling infrastructure has been found to be more effective than the limited mobility of bicycles. People's behavior and choices are affected primarily by the quality and efficiency of transport services provided, rather than by mobility costs, and the need to combine pricing policies with urban and planning procedures to change mobility behaviors and to lead to more sustainable behaviors. The paper concluded with a set of recommendations on sustainable mobility and land use in an integrated framework.