Infertility of the male factor has gradually become a key issue in the care of infertile couples, and up to 50 percent of all cases of couple infertility have been attributed to male factor.(1)
Current literature represents growing concern and attempts to retain capacity of fertility. semen cryopreservation is a simple and realistic solution available before any cytotoxic chemotherapy or radiotherapy for patients with cancer who wish to maintain fertility capacity. It is an important way to preserve reproductive ability in patients facing a possible risk of permanent and longstanding azoospermia.(2)
It is well known that cryopreservation also decreases the motility of sperm, mostly due to osmotic and thermal injury to these cells. During the freeze-thaw process, chromatin stability also decrease, with implication for DNA integrity. This was confirmed by Donnelly et al.,(3) who demonstrated an increase in the fragmentation of single-stranded DNA during cryopreservation of human sperm.
AIM OF THE WORK:
The aim of this work was to evaluate the effect of cryopreservation on DNA fragmentation index of sperm in patients with oilgoasthenoteratozoospermia.