Introduction
Inflammatory Bowel Disease is a chronic disease that affects the colon, it has two types which are Crohn's disease and Ulcerative colitis. There are many factors that cause it, one of these factors is “Dysbiosis" which is the change of the gut microbiome contents of bacteria, one of these bacteria is Akkermansia Muciniphila.
Akkermansia Muciniphila
it is a mucin- degrading bacteria using mucin as a sole source of carbon and nitrogen and it has anti-inflammatory effects in human and it is reduced in IBD.
Aim of the work
The aim of this study is to identify Akkermansia muciniphila in the feces of patients with CD and UC to use it as a microbiomarker in discrimination of CD and UC.


Patients
This study will include eighty patients divided as follow:
Group (I): 30 patients with CD
Group (II): 30 patients with UC
Group (III): 20 healthy patients as control.




METHODS
1. Bacteriome study: Molecular techniques
a. DNA extraction: DNA will be extracted from stool samples using fecal DNA extraction Kit.
b. PCR: Quantitative SYBR green real time PCR using for the identification and quantitation of the dominant genera which constitute the core of the Akkermansia muciniphila.