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198682

GC-MS ANALYSIS OF THREE PLANTS ESSENTIAL OILS AND THEIR EFFECT ON BACTERIAL SPOT DISEASE OF TOMATO

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Last updated: 26 Dec 2024

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Abstract

The aim of this present study is to evaluate the antimicrobial activities of three plants essential oils, Nigella (Nigella sativa), Eucalyptus (Cinnamomum camphora) and clove (Syzygium aromaticum) against plant pathogenic bacteria Xanthomonas vesicatoria and their influences on controlling the bacterial spot disease of tomato plants. The chemical composition of the oils was calculated by gas chromatograph/mass spectrometer (GC/MS) analysis. The results revealed that the major constituents of Nigella oil were fatty acid i.e. oleic acid (4.09%%), Fumaric acid (0.13%), Palmitic acid (4.95-1.65 %), Octadec-9-enoic acid (0.53%), myristic acid (0.78%), Cyclohexadecane (0.01%) and Hexanedioic acid bis (2-ethylhexyl) ester (75.02%). In addition, the main components of Eucalyptus oil were D-camphor (40.01%), linalool (20.57%), and cineole (10.86%). The chemical analysis showed also that clove oil contained eugenol (22.94%), eugenyl acetate (16.65%), caryophyllene (10.37 %) and 2-Pyridineethanol (6.08 %). In in vitro bioassay against X.anthomonas vesicatoria, the results demonstrated that among the three tested plant oils, Eucalyptus oil (10 %) recorded the maximum value was (1.73 cm) bacterial inhibition zone followed by Clove oil (1.50 cm). Results also showed spraying plants with the tested essential oils two days before bacterial inoculation recorded the lowest disease index comparing to the treatment where plants were treating with essential oils two days after bacterial inoculation. The obtained results also clearly illustrated that, Peroxidase (PO), polyphenoloxidase PPO and chitinase activities increased as a result of spraying tomato plants with the tested oils. Moreover, protein analysis confirmed that new protein bands with low molecular weight had a progressive relationship with reduction of bacterial spot disease severity on plants treated with the tested oils.

DOI

10.21608/mjapam.2021.198682

Keywords

GC/MS, Essential oils, Bacterial spot, Xanthomonas vesicatoria, Nigella Sativa, Cinnamomum camphora, Syzygium aromaticum

Authors

First Name

G.A.

Last Name

Ahmed

MiddleName

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Affiliation

Plant Pathology Department, Faculty Agriculture, Moshtohor, Benha University. Egypt

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Orcid

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First Name

A.A.

Last Name

El-Sisi

MiddleName

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Affiliation

Plant Pathology Department, Faculty Agriculture, Moshtohor, Benha University. Egypt

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Orcid

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First Name

M. E.

Last Name

Selim

MiddleName

-

Affiliation

Agricultural botany Department., Faculty of Agriculture, Menoufia University. Egypt.

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Volume

6

Article Issue

6

Related Issue

28050

Issue Date

2021-09-01

Receive Date

2021-10-09

Publish Date

2021-09-01

Page Start

27

Page End

41

Print ISSN

2357-0776

Online ISSN

2735-3494

Article File

MJAPAM_Volume 6_Issue 6_Pages 27-41.pdf

PDF . 638.7kB

Link

https://mjpam.journals.ekb.eg/article_198682.html

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https://mjpam.journals.ekb.eg/service?article_code=198682

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Original Article

Type Code

1,417

Publication Type

Journal

Publication Title

Menoufia Journal of Plant Protection

Publication Link

https://mjpam.journals.ekb.eg/

MainTitle

GC-MS ANALYSIS OF THREE PLANTS ESSENTIAL OILS AND THEIR EFFECT ON BACTERIAL SPOT DISEASE OF TOMATO

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Article

Created At

23 Jan 2023