Grapevine (Vitis vinefera L.) one of the most important fruit crops all over the world, especially in tropical regions. The Egyptian grapes are very important both for local consumption and exportation. Grapevine trees are liable to the attack such as of many pathogens such as Uncinula necator. Powdery mildew symptoms can be seen on foliage, fruit, flower parts and canes. Therefore, the present investigation was conducted to study and focused high lights on isolation and identification of the causal agent from different grapes production areas. For reducing the using of fungicides or chemical compounds, many attempts for control the powdery mildew disease of grapes were carried out using biological control agents against the pathogen as well as antioxidants and some specific and recommended fungicides. The percentage of infection as well as severity of infection were significantly higher in upper Egypt (Beni Suif and Elminia governorates) than that occurred in Lower Egypt (Gharbia and Menoufia governorates). The best treatment of biological control was the mixture of both Trichoderma harzianum and T. viride, where the least severity of infection and output of vitis crop. Recommended dose of each fungicide was suitable in controlling the powdery mildew disease infection. the antioxidant Ascorbic acid in 1&2gm/liter was the best treatment, followed by citric acid, but boric acid come in the third rank in this field. Soil fertilization revealed as very effective factors in disease infection. Rising of nitrogen element raised the disease severity and minimized crop productivity, at the same time rising of potassium and phosphorus minimized the disease parameters and raising the fruit crop.