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123027

ADVANCED TECHNIQUES FOR DETECTION OF IMPORTANT SEED-BORNE FUNGAL DISEASES IN SOME CEREAL CROPS

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Last updated: 26 Dec 2024

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Abstract

The Aims of this study were to: The first aim of the present study was to develop seed health methods that would allow reliable and reasonably fast detection of seed-borne fungal diseases such as Pyricularia oryzea in rice, Ustila.go tritici in wheat and Cephalosporium maydis in maize. Many detection methods occur for different seed-borne pathogens, but, few of the minimum requirements for adequate seed tests were satisfied. Preferably, seed health methods should be sensitive, specific, rapid, robust, inexpensive and simple to implement and interpret, in order to fit plant quarantine Some standard seed health procedures were applied for seed-borne fungi detection from the three seed crops. Washing test, Blotter method, Washing after Incubation (modified), Deep Freezing method and Potato Dextrose Agar method (PDA) were used in case of rice seed samples. Embryo Count method and Field experiment were carried out for loose smut detection. Blotter mehtod, Deep Freezing mehtod and Potato Dextrose Yeast Agar method were implimented in inspecting whole seeds and broken one of maize seed samples. Side by side, PCR detection and identification of the previously seed-borne pathogen was comparabley applied to face the disadvantages of the common tests. Nano-metalic particles were involved in to enhance of DNA and focused to compare between some traditional detection methods and advanced PCR-based techniques for detection of seed-borne pathogens in the three major food crops; wheat, rice and maize.

DOI

10.21608/mjapam.2019.123027

Keywords

rice, Wheat, Maize, Pyricularia oryzea, Ustilago tritici, Cephalosporium maydis, seed health testing, DNA extraction, Nanoparticles, PCR assay

Authors

First Name

M. A.

Last Name

Awad

MiddleName

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Affiliation

Prof. of Plant pathology, Fac. of Agric., Menoufia Univ.

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First Name

G. A.

Last Name

Amer

MiddleName

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Affiliation

Prof. of Plant pathology, Fac. of Agric., Menoufia Univ.

Email

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City

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Orcid

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First Name

I. H. El-

Last Name

Abbasi

MiddleName

-

Affiliation

Professor of Plant Pathology, Agric. Res. Center (ARC)

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Volume

4

Article Issue

5

Related Issue

18429

Issue Date

2019-10-01

Receive Date

2020-11-14

Publish Date

2019-10-01

Page Start

225

Page End

226

Print ISSN

2357-0776

Online ISSN

2735-3494

Article File

MJAPAM_Volume 4_Issue 5_Pages 225-226.pdf

PDF . 247.3kB

Link

https://mjpam.journals.ekb.eg/article_123027.html

Detail API

https://mjpam.journals.ekb.eg/service?article_code=123027

Order

4

Type

Original Article

Type Code

1,417

Publication Type

Journal

Publication Title

Menoufia Journal of Plant Protection

Publication Link

https://mjpam.journals.ekb.eg/

MainTitle

ADVANCED TECHNIQUES FOR DETECTION OF IMPORTANT SEED-BORNE FUNGAL DISEASES IN SOME CEREAL CROPS

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Article

Created At

23 Jan 2023