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118012

MANAGEMENT OF LOOSE SMUT DISEASE (USTILAGO TRITICI) AND DETERMINATION OF FUNGICIDES RESIDUES IN WHEAT MATRICES USING QuEChERS METHODOLOGY

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Last updated: 26 Dec 2024

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Abstract

Loose smut of wheat caused by Ustilago tritici is a disease of economic important in Egypt. It is an exclusively seedborne disease and can be controlled by applying pre-sowing dry seed treatment with any of the nature products as black seed oil and cumin oil or gamma rays@ 150, 200, 250 Gy or four systemic fungicides as Premis® 25% FS, Sumi-8® 2%WP, Dividend Extreme® 11.5% FS and Raxil® 2% DS. The present study was carried out during 2016/2017 and 2017/2018 growing seasons and aimed to evolve an efficient method of application of fungicides, nature products and radiation for an economic control of loose smut of wheat and also evaluated for the determination of four azole fungicides residues in wheat and soil samples. The tested fungicides were highly effective in controlling the disease and gave more than 98% disease control with high grain yield while nature products as black seed oil and cumin oil gave 61.2% and 56.6% control, respectively. For determining the fungicides residues, the fungicides were extracted and cleaned using the ‘Quick Easy Cheap Effective Rugged and Safe' (QuEChERS) method and followed by gas chromatographs coupled to electron capture (µECD) detector analysis. The analytical method was evaluated in terms of recoveries, repeatability, limit of quantitation (LOQ) and matrix effects. The recoveries were between 80.12 to 97.85% with relative standard deviation (RSDs) ranged between 6.12-10.65%. Limit of detections ranged from 0.002 to 0.01mg/kg. LOQ for tested fungicides less than or met the maximum residue limits (MRL).

DOI

10.21608/mjapam.2019.118012

Keywords

Wheat, Loose smut, Ustilago tritici, disease control, Fungicides residues

Authors

First Name

M. A.

Last Name

Gad

MiddleName

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Affiliation

Wheat Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza Egypt

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Orcid

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First Name

A. A.A. El-

Last Name

Ghanam

MiddleName

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Affiliation

Pesticide Residues and Environmental Pollution Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Giza, Egypt

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City

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Orcid

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First Name

Dalia E. El-

Last Name

Hefny

MiddleName

-

Affiliation

Pesticide Residues and Environmental Pollution Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Giza, Egypt

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Volume

4

Article Issue

3

Related Issue

17758

Issue Date

2019-06-01

Receive Date

2020-10-10

Publish Date

2019-06-01

Page Start

107

Page End

118

Print ISSN

2357-0776

Online ISSN

2735-3494

Article File

MJAPAM_Volume 4_Issue 3_Pages 107-118.pdf

PDF . 433.9kB

Link

https://mjpam.journals.ekb.eg/article_118012.html

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https://mjpam.journals.ekb.eg/service?article_code=118012

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3

Type

Original Article

Type Code

1,417

Publication Type

Journal

Publication Title

Menoufia Journal of Plant Protection

Publication Link

https://mjpam.journals.ekb.eg/

MainTitle

MANAGEMENT OF LOOSE SMUT DISEASE (USTILAGO TRITICI) AND DETERMINATION OF FUNGICIDES RESIDUES IN WHEAT MATRICES USING QuEChERS METHODOLOGY

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Article

Created At

23 Jan 2023