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117167

EFFICACY OF SOME BIOCONTROL ORGANISMS, ANIMAL MANURES AND FUNGICIDES ON CONTROLLING OF POTATO BLACK SCURF AND STEM CANKER DISEASE

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Last updated: 26 Dec 2024

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Abstract

In the last few years, stem canker and black scurf disease caused by Rhizoctonia solani Kuhn (teleomorph Thanathephorus cucumeris [Frank] Donk) are among different diseases important attacked potato crop under the Egyptian climate conditions. Eight fungal isolates were isolated from diseased plants representing different localities in different governorates i.e. El-Kassasen (Ismailia Gov.), Kafer El-zayat (Gharbia Gov.), El-Katatba (Minofya Gov.), Housh-Essa (Behera Gov.), El-Badrashen (Giza Gov.), Behdal (Minia Gov.), Dairoot (Assiut Gov.) and Shandaweel (Sohag Gov.). The obtained isolates differed in their culture color, number and size of sclerotia. Isolates which isolated from El-Kassasen, Kafer El-zayat, El-Khatatba, El-Badrashen and Behdal, were belonging to AG-3 anastomosis group. Under greenhouse conditions this isolates were tested for their pathogenicity to potato (Lady-Roseta cv.), during summer growing season 2015 and gave high rates of infection to potato plants. In this manner, the isolate which obtained from El-Badrashen (Giza Gov) was the highest aggressive one and causing the highest percentage of stem canker and black scurf. In separate experiments under greenhouse conditions, a number of biocontrol organisms,animal manure extracts and fungicides were evaluated alone in their efficacy to reduce the infection of potato stem canker and black scurf diseases.With regard to bioagents test, Trichoderma harzianum at concentrate 2.0% improving percentages of eyes germination and reduce dead sprouts of potato and decrease of stem canker and black scurf incidence, compared with Bacillus subtilis and algae treatments. While, animal manure test, Pigeons extract at concentrate 2.0%, gave the highest effect on reducing of stem canker and black scurf incidence compared to Chicken extract. In fungicides experiment, Rizolex (Tolchlophos-methyl) when used before soil inoculation give the best effect in reducing of non-germinated eye, dead sprouts and incidence of stem canker and black scurf compared to Monceren (Pencycuron). On the other hand, the effect of different treatments on the content of potato plants of phenols and oxidative enzymes were studied. In this respect T. harzianum, gave the highest content of phenols and activities of oxidative enzymes Peroxidase (PO) and Polyphenoloxidase (PPO).

DOI

10.21608/mjapam.2019.117167

Keywords

R. solani, Pathogenicity test, biocontrol organisms, Animal manures, fungicides and chemical analysis

Authors

First Name

M.A.

Last Name

Khalil

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Affiliation

Plant Pathol. Res. Inst., Agric. Res. Centre, Giza, Egypt

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Orcid

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First Name

Seham S.

Last Name

Ragab

MiddleName

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Affiliation

Plant Pathol. Res. Inst., Agric. Res. Centre, Giza, Egypt

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City

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Orcid

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First Name

Amal M.

Last Name

Ismail

MiddleName

-

Affiliation

Plant Pathol. Res. Inst., Agric. Res. Centre, Giza, Egypt

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Volume

4

Article Issue

2

Related Issue

17671

Issue Date

2019-04-01

Receive Date

2020-10-05

Publish Date

2019-04-01

Page Start

21

Page End

43

Print ISSN

2357-0776

Online ISSN

2735-3494

Link

https://mjpam.journals.ekb.eg/article_117167.html

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https://mjpam.journals.ekb.eg/service?article_code=117167

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Original Article

Type Code

1,417

Publication Type

Journal

Publication Title

Menoufia Journal of Plant Protection

Publication Link

https://mjpam.journals.ekb.eg/

MainTitle

EFFICACY OF SOME BIOCONTROL ORGANISMS, ANIMAL MANURES AND FUNGICIDES ON CONTROLLING OF POTATO BLACK SCURF AND STEM CANKER DISEASE

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Article

Created At

23 Jan 2023