Six varieties of bread wheat were crossed in half diallel analysis. The fifteen F₁'s and their parents were evaluated. The experiment in a randomized complete block design with three replications at the Experimental farm, Faculty of Agriculture, Menoufia University Shebin El – Kom, Egypt during 2020/2021. The results cleared that genotypes, parents and the resultant fifteen crosses mean squares were found to be highly significant for all traits studied. Parent vs. crosses mean squares as an indication to average heterosis over all crosses were found to be highly significant for all traits studied. Variance of general combining ability was found to be highly significant for all traits studied. The parental variety Shandaweel-1 proved to be good general combiner for grain yield per spike and grain yield per plant. Also, Giza-171 was good general combiner in heading date, grain yield per spike. The most desirable SCA effects for grain yield per plant were detected by the seven hybrids i.e. Shandaweel-1 × Giza-171, Shandaweel-1 × Misr-3, Giza-171× Misr-3,  Misr-3 × Sakha-95, Sakha-95  × Sids-1, Sakha-95  × Morocco and  Sids-1 × Morocco. Desirable and significant useful heterosis for grain yield per plant was detected in all 15 F₁'s. The results show the dendogram on the basis of the molecular parameters on two groups and on the basis of the crop characteristics on three main groups and they were not identical, which indicates the existence of morphological and molecular differences between the varieties. The study indicates that the molecular parameters and yield characteristics can be used successfully in estimating the genetic diversity in wheat.