This investigation was carried out at the experimental farm of the faculty of Agriculture, Menoufia Univ. at Shiben El-Kom during to growing the seasons of 2017 and 2018 to study the effect of some magnetized water and biofertilization on the growth, herb yield and essential oil content of Majorana hortensis L. plants. The N₂-fixing bacterial strains of Rizobium, Azospirillium and Azotobacter were used as biofertilizers in current study. The plants were irrigated either with normal or magnetized irrigation water. The magnetized water was obtained by passing the water through magnetron tube of 2 inches diameter and 4000 Gauss's strains. The obtained results showed that the growth and herb yield parameters were markedly  improved as a result of wateringwith magnetic water besides using bio fertilizers (Rhizobium, Azotobacter and Azospirillium). The best results in this respect were obtained by using magnetic water and Azotobacter fertilizer application compared to the control during both experimental season. Furthermore, the highest essential oil content and the percentages of N, P and K were enhanced as well by the combined application of magnetized water and Azotobacter application.