A field traial allowed in completely randomized design with three replications were conducted in the research farm of the agricultural research station of Etay Al-Baroud (old land) and the research farm of the Faculty of Agriculture, Damanhur University, in El-Bostan region, El-Beheira Governorate (newly reclaimed lands) during 2018 and 2019 seasons to evaluate the performance of twelve new genotypes of soybean compared with the three commercial varieties Giza 111, Giza 22 and Crawford. The resistance of genotypes to cotton leaf worm was estimated in the laboratory by raising a fourth instar larvae of cotton leaf worm and leaving them for feeding on the middle leaflet of the third upper trifoliate leaf of the plant for 24 hours, after that the areas that the insect fed on were measured as a percentage of the total area of the leaf (leaf area consumed). The resistance to cotton leaf worm in the field was estimated by vision according to the Smith and Brim 1979 method (defoliation %). The results confirmed that mean square due to genotypes, locations and genotypes x location were highly significant for all studied traits except the locations mean square for number of hairs under binocular field area and branches number /plant in the first season and genotypes x location mean square of number of hairs under binocular field area, branches number /plant, maturity date and 100-seed weight in both seasons as well as number of pods/plant in the second season. All tested genotypes significantly differ in their performances in all studied traits across the two locations and the four genotypes Line 3, Line 105, Line 127 and Line 129 seemed to excellent genotypes for yield and resistance to cotton leaf worm across the two locations. The consumed leaf area caused by cotton leaf worm and oil percentage negatively associated with pubescence density while seed yield positively associated with pubescence density. Seed yield and oil percentage negatively associated with the defoliation value. In general, it can be said that the four soybean genotypes, Line 3, Line 105, Line 127 and Line 129 are promising genotypes that could be sowing in newly reclaimed lands to expand soybean cultivation in Egypt.