Salinity is one of the most important factors facing the expansion of grapevine agricultural production which leads to reducing growth, yield and cluster quality. So, a field experiment was conducted during 2016 and 2017 seasons in a private vineyard situated in Desouk, Kafr El-Sheikh Governorate, Egypt, to evaluate effects of Humic acid at 15g and 20g/vine, potassium silicate at 20g and 40g/vine, and biofertilizer containing three bacterial strains (Azotobacter chroococcum+ Azospirllium lipoferm+ Bacillus megatherium with cell density 1*108 CFU/ g) at 50g/ vine alone or in combinations on growth, fruit quality and yield of "Ruby seedless" grapevines grown under soil salinity conditions. The results revealed that the combination treatments were more effective to alleviate the adverse effects of soil salinity than the individual ones. The combinations treatments among Humic acid, potassium silicate and biofertilizer at low (Humic acid at 15g+ potassium silicate at 20g+ biofertilizer at 50g/ vine) and high (Humic acid at 30g+ potassium silicate at 40g+ biofertilizer at 50g/ vine) concentrations showed a superior effect on vegetative growth parameters such as leaf area, number of leaves per shoot, total chlorophyll, and vine vigor characters as lick cane length, coefficient of wood ripening, pruning's weight, internodes length, and diameter as compared with control. Moreover, caused a significant increase in leaf N P K content, and reduced leaf proline content. Also, it enhanced berry physical parameters (berry diameter, weight and volume of 100 berries) and chemical parameters (SSC%, SSC/acid ratio and anthocyanin content) as well as cluster number, length and weight consequently increased the total yield per feddan. Moreover, these applications reduced soil EC and pH meanwhile, enhanced both soils available NPK and microorganisms activity.