Microalgae are a potential feedstock for a vast renewable products and different applications in biotechnology. This investigation of using poultry waste extracts for growing Anabaena, Chlorella and mixed culture of both showed that 5 g/L poultry waste extract was the most probable concentration for enhancing growth and lipid production. Percentage increase in lipid content over control (BBM) was (94.2, 56.4 and 28.8 %), biomass productivity was (31.61, 51.86 and 42.04 mg/L/day) and lipid productivity was (4.59, 10.53 and 6.94 mg/L/day) for Anabaena, Chlorella and mixed culture respectively. The role of microalgae in bioremediation of poultry waste was effective, maximum reduction percentage of NH₃ was (84.07%) whereas it was   (80.41%) for phosphorus. Microwave pretreatment of harvested cells increased the lipid content to 17.6% in Anabaena, 31% in Chlorella and 23% for mixture. Lipid extraction, esterification, GC-Ms analysis for fatty acid profile, showed that saturated fatty acids in Chlorella andalgal mixture were higher than their corresponding controls. Cetane number of the produced biodiesel was ranged from (53.7 to 56.4) while iodine value was ranged from (63.5 to 88.4). This study revealed that poultry waste extract can be used as a promising ecofriendly microalgae medium for high production of biomass and lipid which in turn produce high quality biodiesel meet the international standards biodiesel.