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115971

A COMPARATIVE STUDY ON THE ESTIMATION OF EXTRACTED PIGMENTS FROM SOME ALGAE USING DIFFERENT SOLVENTS

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Last updated: 26 Dec 2024

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Abstract

Four different solvents were used to study their efficiency on pigments extraction from three different algal species of Chlorella vulgaris, Spirulina platensis and Nannochloropsis oculata. Dimethylsulfoxide, acetone, ethanol and methanol were used in the extraction process. The experiment was performed in both of fresh and oven dried algal samples. Chlorophylls a, b, total chlorophyll and total carotene concentrations were determined spectrophotometrically and by HPLC technique.  The spectrophotometric method revealed that, dimethylsulfoxide surpasses all other used solvents in all species and all pigments in fresh samples except for chlorophyll a of N. oculata, whereas acetone was more effective than the others in fresh and dry sample. For the dried sample of C. vulgaris, methanol gave the maximum extractability with chlorophyll a while chlorophyll b and total chlorophyll were found be maximum in dimethylsulfoxide solvent. In Spirulina, methanol represented the maximum chlorophyll a and total chlorophyll for the dried samples. Total carotene was found to be higher in ethanol solvent with both C. vulgaris and N. oculata but dimethylsulfoxide surpasses the other solvents with S. platensis. HPLC results emphasized that, acetone surpasses all other used solvent in fresh samples and methanol was ideal solvent in dry samples of C. vulgaris.  Dimethylsulfoxide represented the maximum chlorophyll a and total carotene with Spirulina in both samples. Methanol was the best solvent for extraction of all pigments with N. oculata except with β-carotene of dry sample, ethanol was the best solvent.

DOI

10.21608/egyjs.2016.115971

Keywords

<i>Chlorella Vulgaris</i>, <i>Spirulina platensis</i>, <i>Nannochloropsis oculata</i>, Pigments, dimethylsulfoxide, Acetone, Methanol, ethanol, Spectrophotometer, HPLC

Authors

First Name

Abo El-Khair

Last Name

El-Sayed

MiddleName

B.

Affiliation

Fertilization Technology Department, National Research Centre, Cairo-Dokki, Egypt

Email

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City

-

Orcid

-

First Name

Adel

Last Name

Hamed

MiddleName

F.

Affiliation

Botany Department, Faculty of Science, Ain Shams University, Egypt

Email

ad.ali@ut.edu.sa

City

-

Orcid

-

First Name

Mohamed

Last Name

El-Fouly

MiddleName

M

Affiliation

Fertilization Technology Department, National Research Centre, Cairo-Dokki, Egypt

Email

-

City

-

Orcid

-

First Name

Hesham

Last Name

Abd El Fatah

MiddleName

M.

Affiliation

Botany Department, Faculty of Science, Ain Shams University, Egypt

Email

hesham_zaid@sci.asu.edu.eg

City

-

Orcid

-

First Name

Mohamed

Last Name

Hassoub

MiddleName

A.

Affiliation

Fertilization Technology Department, National Research Centre, Cairo-Dokki, Egypt

Email

-

City

-

Orcid

-

Volume

17

Article Issue

1

Related Issue

17545

Issue Date

2016-11-01

Receive Date

2016-04-19

Publish Date

2016-11-30

Page Start

1

Page End

16

Print ISSN

1110-8649

Online ISSN

2735-3826

Link

https://egyjs.journals.ekb.eg/article_115971.html

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https://egyjs.journals.ekb.eg/service?article_code=115971

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Type

Original Article

Type Code

1,365

Publication Type

Journal

Publication Title

Egyptian Journal of Phycology

Publication Link

https://egyjs.journals.ekb.eg/

MainTitle

A COMPARATIVE STUDY ON THE ESTIMATION OF EXTRACTED PIGMENTS FROM SOME ALGAE USING DIFFERENT SOLVENTS

Details

Type

Article

Created At

23 Jan 2023