This research aims to develop and evaluate small prototype machine performance during gin Egyptian cotton in laboratory. This machine helped technicians scholars to study the effect of ginning experimental parameters on fiber cotton quality properties. The independent variables in this study were: four rotation speeds were 0.79 m/s (60 r.p.m) ,1.18 m/s (90 r.p.m), 1.60 m/s(120 r.p.m) , and 2.36 m/s(180 r.p.m), three feeding rates were 30 kg/h, 45 kg/h and 60 kg/h and three values of clearance between two drums of 3 , 4 and 5 mm. Evaluation parameters were : gin stand productivity, lint turn out, seed wastage mass, gin lint losses and ginning efficiency. Also, energy requirement and total cost production were determined. On other hand, a comparative study between development prototype gin machine and the conventional McCarthy gin roller made to evaluate fiber properties. The test results for developed gin stand performance found that, a maximum of ginning efficiency, gin stand capacity, lint turn out percentage were 81.28 %. 32.8 kg, and 43.1 %, respectively . also, maximum of seeds wastage mass was 17.49 kg/h. while, minimum of gin lint losses percentage was 3.5% and minimum of energy requirement was 154.26 kW.h/Mg. On other hand, minimum of total operation cost was 231.40 L.E/Mg while, minimum of criterion function cost was 364.10 L.E/Mg. consequently, the new developed prototype machine was influenced by all of independent variables. Recently, a comparative study between the developed gin stand and the conventional McCarthy gin roller on the fiber quality properties found that the gin stand developed was consider the best for ginning Egyptian seed cotton samples.