Background: Autophagy and Insulin resistance play a crucial role in pathogenesis of T2DM. Novel accurate strategies for detection of molecular pathogenesis of insulin resistance are distinctly needed. Aim of the work: To retrieve potential mRNA associated with insulin resistance related autophagy from databases followed by validation of this network in patients' clinical samples. Material and Methods: Bioinformatics' Analysis was done to retrieve a gene related to autophagy and IR in T2DM, then biomarker' verification was done through Gene Cards Database. Extraction of total RNA from PMNCs followed by reverse transcription PCR, then quantitative real time PCR for mRNA m-TOR in PMNCs of 123 patients with T2DM and 106 healthy normal volunteers. Results: The mRNA m-TOR had high sensitivity and specificity for discriminating T2DM patients from healthy controls. Conclusion: The mRNA m-TOR can serve as a potential biomarker for T2DM diagnosis and prognosis.