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224554

MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES

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Last updated: 04 Jan 2025

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Abstract

The goal of this work was to describe the sequences of some candidate genes (IGF-I, IGF-I receptor, and Leptin) that are associated with economically important quantitative aspects in dairy buffalo, such as reproductive and productive attributes, as well as milk composition. Ninety-nine dairy buffaloes were used to compare the pure Egyptian buffalo (PE) with the Egyptian–Italian crossbred G1 (25.0%), G2 (50.0%), G3 (62.5%), G4 (75.0%), G5 (87.5%), and G6 (94.0%), respectively. All buffaloes investigated were genotyped BB, which means they were negative for the SnaBI at position 224^225 (TAC^GTA) of the IGF-I regulatory region, and they were genotyped AA-positive for the IGF-I receptor TaqI at position 47^48 (T^CGA). They also tested positive for the leptin gene's Alu1 restriction site yielding three products with genotype GT that was 55-, 118-, and 205-bp in length (AG^CT). Finally, the PE and Egyptian-Italian crossbred demonstrate monomorphism since the two Bubaline populations are closely related and the genes in question are maintained. More research is needed to learn more about Egyptian-Italian buffalo crossbreeds before national crossbreeding initiatives may be expanded.

DOI

10.21608/ejap.2022.114631.1032

Keywords

Egyptian-Italian buffalo, Insulin-like growth factor, leptin, Restriction fragment length polymorphism

Authors

First Name

sarah

Last Name

Ali

MiddleName

Gamal

Affiliation

department of biotechnology, animal production research institute

Email

sarahgamalali2009@gmail.com

City

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Orcid

-

First Name

alia

Last Name

elsaudi

MiddleName

Ahmed

Affiliation

Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.

Email

alia_elsaudi@agr.asu.edu.eg

City

-

Orcid

-

First Name

Ayman

Last Name

Saeed

MiddleName

Moustafa

Affiliation

Department of Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt.

Email

aymansm@arc.sci.eg

City

-

Orcid

-

First Name

Asmaa

Last Name

Abushady

MiddleName

Mohammed

Affiliation

Biotechnology school, Nile University, Sheikh Zayed, Giza, Egypt. Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.

Email

aabushady@nu.edu.eg

City

-

Orcid

-

Volume

59

Article Issue

1

Related Issue

32132

Issue Date

2022-02-01

Receive Date

2022-01-11

Publish Date

2022-02-01

Page Start

9

Page End

18

Print ISSN

0302-4520

Online ISSN

2735-3028

Article File

EJAP_Volume 59_Issue 1_Pages 9-18.pdf

PDF . 1.6MB

Link

https://ejap.journals.ekb.eg/article_224554.html

Detail API

https://ejap.journals.ekb.eg/service?article_code=224554

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2

Type

Original Article

Type Code

1,298

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Production

Publication Link

https://ejap.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES

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Article

Created At

22 Jan 2023